LH、FSH对初情期母牦牛卵巢发育的影响

王志强，张洪波，张君^*   徐尚荣，彭巍，杨启林

（腾博会官网诚信为本，西宁，810016）

摘要：用Elisa对3头初情期内发情母牦牛和7头未发情的初情期母牦牛进行24h内血液促黄体生成素（Luteotropic hormone，LH）、卵泡刺激素（follicle-stimulating hormone，FSH）分泌情况的检测并通过外表观察及组织切片制作，对比发情母牦牛与未发情母牦牛卵巢生长发育的情况。结果显示，发情期24h内，发情母牦牛血液LH、FSH分泌量明显高于未发情母牦牛(p<0.01)；LH呈现明显的脉冲式分泌，而FSH则无脉冲分泌现象。发情母牦牛卵巢质量、横径均大于未发情母牦牛，且发情母牦牛多为初级卵泡、次级卵泡，成熟卵泡数量较少；而未发情母牦牛多见初级卵泡、无核卵泡。

关键词：GnRH； LH；初情期母牦牛； 卵巢

中图分类号：S857.2      文献标识码：A     文章编号：1003-7950（2017）02-0024-05

Effect of LH and FSH on ovaries development of pubertal female yak 

WANG Zhi-qiang etal

（Qinghai Academy of Animal and Veterinary Science,Qinghai  UNIVERSITY,Xining,810016 ）

Abstract: The secretion patterns of Luteotropic hormone(LH) and follicle-stimulating hormone(FSH) of three estrous and seven non-estrous pubertal female yaks were measured by using Elisa during 24 hours, and compare ovary’s growth and development situation between estrous and non-estrous pubertal female yaks through observation and tissue slice technology. The results indicated that the secretion levels of estrus female yak’s LH、FSH was higher than non-estrous female yak (P<0.001) during estrus’s 24 hours, LH presents impulse secretion, but FSH presents no impulse secretion. The ovaries quality, transverse diameter of estrus female yak’s were higher than non-estrous female yaks, and estrus female yak’s ovary mainly exist primary follicle、secondary follicle, but mature follicle rarely can been seen. Non-estrous female yak exist mainly primary follicle, seedless follicle.

Keywords：GnRH; LH; Pubertal female yak; Ovary