荒漠草原优势植物根际溶磷菌溶磷能力研究

张英, 李宗仁，李希来，谢永丽 , 芦光新，杨帆

(青海大学农牧学院草业科学系, 三江源生态与高原农牧业国家重点实验室，三江源区高寒草地生态省部共建教育部重点实验室，青海 西宁　810016)

摘要：本实验以西藏阿里地区优势植物根际溶磷菌为研究对象，利用平板培养基和选择性培养基对来自不同植物的菌株的溶磷能力进行初步测定，其中六株在蒙金娜有机磷培养基上形成明显透明圈,其D/d值(溶磷圈直径与菌落直径比值)在1.944~2.326之间; 五株具有溶解无机磷能力，其D/d值在1.164~1.298之间；1株菌株既能溶解有机磷又可溶解无机磷。对所有菌株进行液体培养，用钼蓝比色法测定有效磷增量分别为8.933~20.932μg/mL和10.981~36.220μg/mL。PWXZ6和NXZ4溶解无机磷能力较其他菌株强，NXY18溶解有机磷能力较其他菌株强。结果表明：PWXZ6、NXZ4和NXY18在后续研制微生物肥料时有较大的潜力。

关键词：根际；溶磷菌；溶磷能力；PKO；蒙金娜[1]

中图分类号：S812    文献标识码：A    文章编号：1003-7950（2007）02-0001-05

 

Primary research of dissolved phosphorus bacteria of dominant plant

 rihizosphere dissolving phosphorus capability in desert grassland

 ZHANG Ying etal

(Grassland Department , Agriculture and Animal Husbandry College, Qinghai University,810016)

 Abstract: Phosphate dissolving bacteria from different dominant plant Rihizosphere in Ali region of Tibet were screed and their phosphate dissolving ability were checked primarily by using petri medium and selectivity medium, six strains were presented obvious transparence circle on Mendajin organic phosphorus medium that D/d value is between 1.164~1.298; Five strains owned phosphate dissolving ability that D/d value is between 1.164~1.298. One strain dissolved not only organic phosphorus but also inorganic phosphorus. The strains were cultured in liquid medium, effective phosphorus quantity were increased by 8.933~20.932 and 10.981~36.220μg/mL respectively by using molybdenum-blue colorimetry. The results showed that the strains of PWXZ6,NXZ4 has better dissolved inorganic phosphorus ,and NXY18 has better dissolved organic phosphorus. So the three kinds of strains have better potential for further studying

microbe fertilizer and development.

 

Key words: Rihizosphere; Soluble phosphorus bacteria; Soluble phosphorus ability; PKO; Menjinna